The Maserati brothers, Alfieri, Bindo, Carlo, Ettore, and Ernesto were all involved with automobiles from the beginning of the 20th century. Alfieri, Bindo and Ernesto built 2-litre Grand Prix cars for Diatto. In 1926, Diatto suspended the production of race cars, leading to the creation of the first Maserati and the founding of the Maserati marque. One of the first Maseratis, driven by Alfieri, won the 1926 Targa Florio. Maserati began making race cars with 4, 6, 8 and 16 cylinders (two straight-eights mounted parallel to one another). Another Maserati brother, Mario, an artist, is believed to have devised the company's trident emblem, based on the Fontana del Nettuno, Bologna.

Alfieri Maserati died in 1932, but three other brothers, Bindo, Ernesto and Ettore, kept the firm going, building cars that won races.